Polymeric nanocapsules for the ocular delivery of siRNA

  1. Mendes Saraiva, Sofia
Dirixida por:
  1. María José Alonso Fernández Director

Universidade de defensa: Universidade de Santiago de Compostela

Fecha de defensa: 29 de novembro de 2019

  1. María del Rocío Herrero Vanrell Presidente/a
  2. Carmen Álvarez Lorenzo Secretario/a
  3. Helena Isabel Fialho Florindo Roque Ferreira Vogal

Tipo: Tese


The development of siRNA-based therapies for the treatment of severe ocular diseases is being proposed as a promising therapeutic option for the treatment of severe ocular diseases. Unfortunately, the capacity of siRNA molecules to overcome the multiple protective barriers of the eye is very limited. The specific objective of this work was to develop multiplayer protamine NCs loading high amounts of siRNA (up to 5 mg/mL) for ocular topical administration. Different preparation methods and compositions were studied for the development of NCs complying with the requirements of drug loading and release and also in terms of stability. Among the multiple formulations developed, one of them led to acceptable silencing results in vitro and showed the ability to interact efficiently with the rabbits’ corneal epithelium.