In situ analysis of the antibacterial activity of the essential oils on the oral biofilm

  1. Quintas González, Víctor
Dirixida por:
  1. Inmaculada Tomás Carmona Director

Universidade de defensa: Universidade de Santiago de Compostela

Fecha de defensa: 12 de xaneiro de 2018

  1. Marta Mendonça Moutinho Relvas Presidente/a
  2. Pablo Ignacio Varela Centelles Secretario
  3. Yolanda Jiménez Soriano Vogal

Tipo: Tese


The aim of this thesis was to evaluate the in situ antibacterial activity (immediate effect and substantivity) of a single mouthwash of T-EO, compared to the activity of the 0.2% chlorhexidine (0.2% CHX, positive control). This same comparison was done once again for the analysis of the antiplaque effect on a biofilm model of 4 days. Afterwards, these two comparisons were repeated, but this time assessing the effects of the Af-EO solutions vs. the T-EO solution. A single mouthwash of the traditional formulation of EO presented very high immediate antibacterial effect in situ and a substantivity which lasted for at least 7 hours after its application over a 2-day PL-biofilm. These results were even better than those observed with 0.2% CHX under the same conditions. The T-EO and 0.2% CHX showed a very high antiplaque effect. Although the 0.2% CHX showed better results with regard to reducing the thickness and covering grade by the biofilm, both antiseptics showed a high and similar antibacterial activity. Both EO antiseptics had very high immediate antibacterial activity and substantivity in situ on the 2-day PL-biofilm after a single mouthwash. In the 4-day PL-biofilm, both EO formulations demonstrated a very high antiplaque effect in situ, although the alcohol-free formula performed better at reducing the biofilm thickness and CG.