New near ultraviolet laser-induced native fluorescence detection coupled to HPLC to analyse residues of oxolinic acid and flumequinea comparison with conventional xenon flash lamp

  1. Feás Sánchez, Xesús
  2. Fente Sampayo, Cristina A.
  3. Hosseini, Seyed Vali
  4. Cepeda, A.
Journal:
CyTA: Journal of food

ISSN: 1947-6337 1947-6345

Year of publication: 2009

Volume: 7

Issue: 1

Pages: 15-21

Type: Article

DOI: 10.1080/11358120902850552 DIALNET GOOGLE SCHOLAR

More publications in: CyTA: Journal of food

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Abstract

A new high-performance liquid chromatography (HPLC) method is described for the determination of oxolinic acid and flumequine, with ultraviolet laser-induced fluorescence detection (UV-LIFD). Near-UV excitation at 325 nm was obtained by using an He/Cd laser. Data obtained using UV-LIFD and conventional fluorimetry (Xenon flash, ?exc 325/ ?em 365) are compared under the same chromatographic conditions, connecting in series both detectors, in terms of linearity, reproducibility and repeatability. The HPLC separation is carried out on a Synergi MAX-RP column with water-acetonitrile (2:1, v/v) adjusted at pH 2.5, with formic acid, as mobile phase and completed in less than 9 min. The detection limits of oxolinic acid and flumequine at a signal-to-noise ratio of 3 were 0.43 pg and 0.76 pg on column with UV-LIFD detection, making this method considerably more sensitive than traditional fluorescence detector (16.15 pg and 14.17 pg) having some obvious advantages.