Células madre pluripotentes inducidasgeneración, caracterización y aplicaciones en el estudio y tratamiento de la artrosis

Abstract

Osteoarthritis is the most prevalent joint disease and the main cause of quality life deterioration. However, there are no effective drugs or treatments promoting the restoration of articular cartilage injuries. The discovery of induced pluripotent stem cells or iPSCs has enabled researches with a promising cell source for developing biomedical studies. Due to its intrinsic properties, iPSCs represent an invaluable tool for disease modelling, regenerative medicine and tissue engineering applications. The aim of this research has been, firstly, to generate and characterize iPSCs obtained from patients with hand osteoarthritis, in order to establish cellular models of the disease. Secondly, more focused on tissue engineering approaches, we sought to deeply study iPSCs chondrogenic differentiation aiming to generate cartilage-like tissue in vitro by using 3D printing techniques. Results obtained shown the successful generation of two iPSC-lines from patients with osteoarthritis and one iPSC-line from a healthy donor. These cell lines presented different chondrogenic capacities and therefore, they could be used as a tool to improve our knowledge regarding the molecular and cellular mechanisms causing osteoarthritis development. Moreover, we shown that iPSCs are able to chondrogenically differentiate after 3D bioprinting to generate cartilage-like tissue in vitro.