Estudio piloto de tolerabilidad cutánea de láminas de fibrina-agarosa en voluntarios sanos

  1. Fernández González, Ana
  2. Lizana Moreno, Antonio Manuel
  3. Fernández Porcel, Natividad
  4. Guerrero Calvo, Jorge
  5. Ruiz García, Antonio
  6. Espinosa Ibáñez, Olga
  7. Sierra Sánchez, Álvaro
  8. Ordóñez Luque, Alexandra
  9. Arias Santiago, Salvador
Journal:
Actualidad médica

ISSN: 0365-7965

Year of publication: 2016

Tome: 101

Issue: 799

Pages: 171-175

Type: Article

DOI: 10.15568/AM.2016.799.OR04 DIALNET GOOGLE SCHOLAR lock_openDIGIBUG editor

More publications in: Actualidad médica

Sustainable development goals

Abstract

Purpose: This study aims to analyse possible adverse reactions resulting from the topical use of fibrin-agarose substitutes in the forearm of healthy volunteers. Methods: An experimental study was carried out in seven healthy volunteers, five males and two females, who did not have any cutaneous lesion. Two fibrin-agarose substitutes of 4 cm2 were placed in the forearm of each volunteer. Each substitute was covered with an impregnated dressing and one of the substitutes was covered with antibiotic ointment (mupirocin). Both substitutes were finally covered with a protective dressing. The substitutes were maintained for 48 hours. Results: The results determined that no adverse reactions were detected in any volunteer after 48 hours and a week of evolution. Differences were observed between the two substitutes implanted in each volunteer, since when removing the covered dressing with antibiotic ointment, the substitute presented a more hydrated appearance than the one without antibiotic cream. Conclusions: The implant of fibrin-agarose substitutes in healthy volunteers does not present irritation or allergic type adverse reactions when they applied directly topically on the skin. Although the sample size is low, the fibrin-agarose combination is presented as the biomaterial suitable for the development of an artificial human skin model.

Bibliographic References

  • Ahmed TAE, Dare E V, Hincke M. Fibrin: a versatile scaffold for tissue engineering applications. Tissue Eng Part B Rev. 2008;14(2):199–215.
  • Alaminos M, Sánchez-Quevedo MDC, Muñoz-Ávila JI, Serrano D, Medialdea S, Carreras I, et al. Construction of a complete rabbit cornea substitute using a fibrin-agarose scaffold. Investig Ophthalmol Vis Sci. 2006;47(8):3311–7.
  • Bell E, Ehrlich HP, Buttle DJ, Nakatsuji T. Living tissue formed in vitro and accepted as skin-equivalent tissue of full thickness. Science. 1981;211(4486):1052–4.
  • Bhat ZF, Bhat H, Pathak V. Principles of Tissue Engineering. Principles of Tissue Engineering. 2014. 1663-1683 p.
  • Biradar S, Goornavar V, Periyakaruppan A, Koehne J, Hall JC, Ramesh V. Agarose gel tailored calcium carbonate nanoparticles-synthesis and biocompatibility evaluation. J Nanosci Nanotechnol. 2014;14(6):4257–63.
  • Black AF, Bouez C, Perrier E, Schlotmann K, Chapuis F, Damour O. Optimization and Characterization of an Engineered Human Skin Equivalent. Tissue Eng. 2005;11(5- 6):723–33.
  • Burnouf T, Goubran HA, Chen T-M, Ou K-L, El-Ekiaby M, Radosevic M. Blood-derived biomaterials and platelet growth factors in regenerative medicine. Blood Rev. 2013 Mar;27(2):77–89.
  • Burnouf T, Su C-Y, Radosevich M, Goubran H, El-Ekiaby M. Blood-derived biomaterials: fibrin sealant, platelet gel and platelet fibrin glue. Isbt Sci Ser Vol 4, No 1, State Art Present. 2009;4:136–42.
  • Carriel V, Garzón I, Jiménez JM, Oliveira ACX, Arias-Santiago S, Campos A, et al. Epithelial and stromal developmental patterns in a novel substitute of the human skin generated with fibrin-agarose biomaterials. Cells Tissues Organs. 2012;196(1):1–12.
  • Cen L, Liu W, Cui L, Zhang W, Cao Y. Collagen tissue engineering: Development of novel biomaterials and applications. Pediatric Research. 2008. p. 492–6.
  • Chevallay B, Herbage D. Collagen-based biomaterials as 3D scaffold for cell cultures: applications for tissue engineering and gene therapy. Med Biol Eng Comput. 2000;38(2):211–8.
  • De J, Cardona C, Ionescu AM, Go R, Gonza M, Campos A, et al. Transparency in a fibrin and fibrin-agarose corneal stroma substitute generated by tissue engineering. Cornea. 2011;30(12):1428–35.
  • Garzón I, Martín-Piedra MA, Alfonso-Rodríguez C, González-Andrades M, Carriel V, Martínez-Gómez C, et al. Generation of a biomimetic human artificial cornea model using wharton’s jelly mesenchymal stem cells. Investig Ophthalmol Vis Sci. 2014;55(7):4073–83.
  • Garzón I, Sánchez-Quevedo MC, Moreu G, GonzálezJaranay M, González-Andrades M, Montalvo A, et al. In vitro and in vivo cytokeratin patterns of expression in bioengineered human periodontal mucosa. J Periodontal Res. 2009;44(5):588–97.
  • Garzon I, Serrato D, Roda O, Del Carmen Sánchez-Quevedo M, González-Jaranay M, Moreu G, et al. In vitro cytokeratin expression profiling of human oral mucosa substitutes developed by tissue engineering. Int J Artif Organs. 2009;32(10):711–9.
  • Glowacki J, Mizuno S. Collagen scaffolds for tissue engineering. Biopolymers. 2008;89(5):338–44.
  • Grassl ED, Oegema TR, Tranquillo RT. Fibrin as an alternative biopolymer to type-I collagen for the fabrication of a media equivalent. J Biomed Mater Res. 2002;60(4):607–12.
  • Ionescu AM, Alaminos M, Cardona J de la C, García-López Durán J de D, González-Andrades M, Ghinea R, et al. Investigating a novel nanostructured fibrin-agarose biomaterial for human cornea tissue engineering: Rheological properties. J Mech Behav Biomed Mater. 2011;4(8):1963–73.
  • Ionescu AM, Cardona JC, Garzón I, Oliveira AC, Ghinea R, Alaminos M, et al. Integrating-sphere measurements for determining optical properties of tissue-engineered oral mucosa. J Eur Opt Soc. 2015;10.
  • Jockenhoevel S, Zund G, Hoerstrup SP, Chalabi K, Sachweh JS, Demircan L, et al. Fibrin gel advantages of a new scaffold in cardiovascular tissue engineering. Eur J Cardiothoracic Surg. 2001;19(4):424–30.
  • Lamme EN, Van Leeuwen RTJ, Brandsma K, Van Marie J, Middelkoop E. Higher numbers of autologous fibroblasts in an artificial dermal substitute improve tissue regeneration and modulate scar tissue formation. J Pathol. 2000;190(5):595–603.
  • Langer R, Vacanti JP. Tissue engineering. Science. 1993;260(5110):920–6.
  • Larouche D, Paquet C, Fradette J, Carrier P, Auger FA, Germain L. Regeneration of skin and cornea by tissue engineering. Methods Mol Biol. 2009;482:233–56.
  • Lee CH, Singla A, Lee Y. Biomedical applications of collagen. Int J Pharm. 2001;221(1-2):1–22.
  • Li Y, Meng H, Liu Y, Lee BP. Fibrin gel as an injectable biodegradable scaffold and cell carrier for tissue engineering. Scientific World Journal. 2015.
  • MacNeil S. Progress and opportunities for tissueengineered skin. Nature. 2007;445(7130):874–80.
  • Miyata S, Tateishi T, Ushida T. Influence of cartilaginous matrix accumulation on viscoelastic response of chondrocyte/agarose constructs under dynamic compressive and shear loading. J Biomech Eng. 2008;130(5):051016.
  • Parenteau-Bareil R, Gauvin R, Berthod F. Collagen-based biomaterials for tissue engineering applications. Materials (Basel). 2010;3(3):1863–87.
  • Rodríguez IA, López-López MT, Oliveira ACX, SánchezQuevedo MC, Campos A, Alaminos M, et al. Rheological characterization of human fibrin and fibrin-agarose oral mucosa substitutes generated by tissue engineering. J Tissue Eng Regen Med. 2012;6(8):636–44.
  • Sanchez-Quevedo MC, Alaminos M, Capitan LM, Moreu G, Garzon I, Crespo P V., et al. Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering. Histol Histopathol. 2007;22(4-6):631– 40.
  • Scarano A, Carinci F, Piattelli A. Lip augmentation with a new filler (agarose gel): a 3-year follow-up study. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology. 2009.
  • Selmi T a S, Verdonk P, Chambat P, Dubrana F, Potel J-F, Barnouin L, et al. Autologous chondrocyte implantation in a novel alginate-agarose hydrogel: outcome at two years. J Bone Joint Surg Br . 2008;90(5):597–604.
  • Shakya AK, Holmdahl R, Nandakumar KS, Kumar A. Polymeric cryogels are biocompatible, and their biodegradation is independent of oxidative radicals. J Biomed Mater Res Part A. 2014;102(10):3409–18.
  • Supp DM, Boyce ST. Engineered skin substitutes: Practices and potentials. Clin Dermatol. 2005;23(4):403–12.
Data source: Dialnet