Análisis de la secuencia de nucleótidos del gen de la capside y la polimerasa entre diferentes aislamientos del virus motoso del enanismo de la frambuesa
- Ángel, J.
- Mayo, M.
ISSN: 0123-3475
Year of publication: 1998
Volume: 1
Issue: 2
Pages: 40-45
Type: Article
More publications in: Revista Colombiana de Biotecnología
Abstract
This work was aimed to find sequence variability between capsid and polymerase gene sequences of five RBDV samples. Capsid and polymerase cDNAs were obtained by reverse transcription and PCR (RT-PCR) of RNA extracted from plants inoculated with each of the respective isolated samples. The amplified products were cloned in pGEM-T and sequenced. The results showed that the capsid and polymerase sequences varied less than 1% among the isolated samples. These data suggested that capsid and polymerase transgenic sequences that protect against a particular RBDV sample might protect against the others.