Técnica de cultivo de mioblastos autólogos humanos para transplante clínico
- María Esther Rendal Vázquez
- M. Rodríguez
- T. Díaz
- Alberto Juffé Stein
- N. Domenech
- B. Adrio
- MJ Sánchez
- Cándido Andión Núñez
- F.J. Blanco
ISSN: 1888-6116
Year of publication: 2008
Volume: 19
Issue: 1
Pages: 62-67
Type: Article
More publications in: Trauma
Abstract
Introduction: Cellular transplant to repair or regenerate damaged myocardium is a new objective in cardiovascular disease. The autologous skeletal myoblasts are the most studied cells and constitute the first election for cardiac repair. Objectives: fine adjustment of the cellular cardiomyoplasty technique with revascularization in two patients. Material and methods: 15 biopsies were obtained from multiorganic donors and from two patients with no recent infarct. After three weeks of culture, the percentage of myoblasts were evaluated using monoclonal antibodies CD56, desmin and myogenin. The two patients were subjected to revascularization surgery and intramyocardic injection of autologous skeletic myoblasts obtained after culture with autologous serum. Results: The presence of a great number of positive cells with desmin and myogenin markers was shown. The implantation of autologous skeletal myoblasts was not associated with the development of adverse effects. Conclusions: In patients without a recent myocardium infarct, the treatment with myoblasts together with coronary artery bypass is sure and easy and it is straightforward to obtain myoblasts from muscle tissue for transplant