Nanoengineered delivery of RNAimpact on brain diseases and COVID-19 vaccination

  1. López Borrajo, Mireya
Dirixida por:
  1. María José Alonso Fernández Director

Universidade de defensa: Universidade de Santiago de Compostela

Fecha de defensa: 22 de decembro de 2023

Tribunal:
  1. Bruno Filipe Carmelino Cardoso Sarmento Presidente/a
  2. María Dolores Torres López Secretario/a
  3. Maria Jesús Vicent Docon Vogal

Tipo: Tese

Resumo

Genetic therapies show great potential for treating various diseases through modulation of the expression of specific genes. Their effective delivery is crucial, particularly for challenging conditions like CNS diseases and SARS-CoV-2 vaccines. Here, we used nanotechnology as a fundamental tool for RNA delivery. First, we engineered an ionizable nanoemulsion designed for brain diffusion and delivery of RNA. Second, we explored nanocarriers loaded with siRNA for brain delivery, exploring their performance following alternative administration routes. Finally, we encapsulated mRNAs derived from SARS-CoV-2 within nanoemulsions and polymeric nanocapsules, achieving significant cellular immune responses.