Demostración Inmunohistoquímica de Células de Langerhans en Córnea Humana
- Sedano Gelvet, Eduardo
- Victorio, John
- Neira, Carlos
- Rojas, César
ISSN: 1025-5583, 1609-9419
Year of publication: 1997
Volume: 58
Issue: 3
Pages: 182-188
Type: Article
More publications in: Anales de la Facultad de Medicina
Abstract
We have achieved the identification of Langerhans cells in human cornea epithelium cross sections, fixed in Zenker’s liquid and embedded in paraffin, according to streptavidin-biotin peroxidase immunohistochemical method, in order to demonstrate protein S-100 and muramidase (lisozime) presence in them.Langerhans cells were identified easily from the rest of epithelial cells because they were the only elements protein S-100+ and muramidase+, which were stained light brown and showed rounded outlines with one or two prolongations.Langerhans cells were numerous in the limbic zone and null in the central region.