Demostración Inmunohistoquímica de Células de Langerhans en Córnea Humana

1. Sedano Gelvet, Eduardo
2. Victorio, John
3. Neira, Carlos
4. Rojas, César

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DOI: 10.15381/ANALES.V58I3.4674 DIALNET GOOGLE SCHOLAR lock_openDialnet editor

Outras publicacións en: Anales de la Facultad de Medicina

Resumo

We have achieved the identification of Langerhans cells in human cornea epithelium cross sections, fixed in Zenker’s liquid and embedded in paraffin, according to streptavidin-biotin peroxidase immunohistochemical method, in order to demonstrate protein S-100 and muramidase (lisozime) presence in them.Langerhans cells were identified easily from the rest of epithelial cells because they were the only elements protein S-100+ and muramidase+, which were stained light brown and showed rounded outlines with one or two prolongations.Langerhans cells were numerous in the limbic zone and null in the central region.

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